Coding

Part:BBa_K2622005

Designed by: Auksė Gai�auskaitė   Group: iGEM18_Vilnius-Lithuania   (2018-10-10)


GFP Nanobody

A disulphide bond - free GFP nanobody that binds to GFP (Kd(nM)=0.13). Its expression levels in E. coli are recognized to be very low - 20µg/L of E. coli culture. This biobrick has RFC10 prefix and suffix and dinucleotides - AT at the start of the part and GC at the end of the part that are used for protein fusion with RFC12 biobricks in order to avoid ORF changes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None